South China Agricultural University/Guangdong Provincial Key laboratory of Food Safety and Quality,South China Agricultural University/Guangdong Provincial Key laboratory of Food Safety and Quality,South China Agricultural University/Guangdong Provincial Key laboratory of Food Safety and Quality,South China Agricultural University,South China Agricultural University/Guangdong Provincial Key laboratory of Food Safety and Quality,South China Agricultural University/Guangdong Provincial Key laboratory of Food Safety and Quality,South China Agricultural University/Guangdong Provincial Key laboratory of Food Safety and Quality
This work was supported by grants from The National Natural Science Foundation of China (31271865, 31301467), The National Basic Research Program of China (2012CB720803) and The Natural Science Foundation of Guangdong Province, China (S2012040008046)
Immunoassay based on antigen-antibody interaction plays an important role in the screening of low molecular mass organic contaminations such as pesticides, veterinary drugs and biological toxins. However, due to the lack of epitope groups of haptens, the production of antibodies with high affinity is still one of the bottlenecks of immunoassay for small molecules. Dendrimers, as a new type polymer, exhibit many excellent structural properties and histocompatibility, such as clear molecular composition, regular, highly branched and nanometer-sized structure, monodisperse property and presenting dense functional groups on its surface. Previous studies had revealed that dendrimers are potential in the improving performance of immunoassay, such as to produce antibodies with higher affinity, produce antibodies with broad-specificity and improve assay sensitivity. In this work, therefore, the application of dendrimers as carrier to prepare immunogens and coating antigens, as adjuvant to improve immunogenicity, as carrier to amplify signal were reviewed. The potential of application of dendrimers on the development of immunoassays for small molecules was also discussed.
LIU Feng-Yin, SHEN Yu-Dong, SUN Yuan-Ming, WANG Hong, LEI Hong-Tao, YANG Jin-Yi, XU Zhen-Lin. Progress on The Application of Dendrimers to Improve Performance of Immunoassays[J]. Progress in Biochemistry and Biophysics,2014,41(7):649-658
Copy® 2025 All Rights Reserved ICP:京ICP备05023138号-1 京公网安备 11010502031771号