Tip60 (KAT5), a member of MYST acetyltransferase family, is an important component of an evolutionarily conserved complex, NuA4. In the past ten years, a number of functions have been discovered for Tip60. It could act as transcriptional regulation factor together with or without nuclear receptors to activate or inhibit downstream gene expression; or acetylate a series of proteins to regulate their activity and stability. Through its acetyltransferase activity, Tip60 has been shown to regulate some important signaling pathways, such as DNA damage repair response, cell cycle, checkpoint activation, apoptosis, metabolism and autophagy. In addition, Tip60 also plays a crucial role in the development and metastasis of tumor and embryonic development. Here, we summarized the functions of Tip60 in recent years.