Analysis of Phosphatidylcholines (PCs) and Lysophosphatidylcholines (LysoPCs) in Metastasis of Breast Cancer Cells

Home > Archive>Volume 42, Issue 6, 2015 >563-573

Analysis of Phosphatidylcholines (PCs) and Lysophosphatidylcholines (LysoPCs) in Metastasis of Breast Cancer Cells
DOI:
                        
Author:
                        
Affiliation:State Key Laboratory of Medicinal Chemical Biology,Department of Biochemistry,College of Life Sciences,Nankai University,Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P.R. China,State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071, China,State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071, China,State Key Laboratory of Medicinal Chemical Biology, Department of Cancer Research, College of Life Sciences, Nankai University, Tianjin 300071, China,State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071, China,State Key Laboratory of Medicinal Chemical Biology, Department of Biochemistry, College of Life Sciences, Nankai University, Tianjin 300071, China
Clc Number:
Fund Project:This work was supported by grants from Tianjin Natural Scientific Foundation (14JCZDJC32800), National Basic Research Program of China (2015CB553905), Hi-Tech Research and Development Program of China (2014AA020903) and The National Natural Science Foundation of China (81372186)

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

The important role of phospholipids in many processes including cell cycle regulation, inflammation and tumorigenesis is increasingly becoming the focus of research. Previously we found that cytosolic phospholipase A2 (cPLA2) is over-expressed in breast cancer cells. Here, we assessed the profiles of phosphatidylcholines (PCs) and lysophosphatidylcholines (LysoPCs) that were mediated by cPLA2 in breast cancer cells. MCF-7 and LM-MCF-7 (with high metastatic potential) cell lines were used as models and the profiles of phospholipids were analyzed by HPLC/ESI/MSⁿ. Our results showed that the levels of 10 species of LysoPCs were higher in LM-MCF-7 cells than that in MCF-7 cells. Meanwhile, six species of PCs that may produce arachidonic acid (AA) and associated LysoPCs were lower in LM-MCF-7 cells than that in MCF-7 cells. Furthermore, we identified that 4 out of 6 species of PCs were responsible for the generation of AA mediated by cPLA2 in LM-MCF-7 cells. The increased ratio of LysoPCs to PCs corresponds to the activation level of cPLA2. Thus, we conclude that 4 identified species of PCs and 4 corresponding species of LysoPCs mediated by cPLA2, as well as the up-regulation and activation of cPLA2, may involve in the metastasis of breast cancer cells.

Reference

Cited by

Get Citation

YOU Jia-Cong, YANG Jie, FANG Run-Ping, HU Nan, ZHANG Xiao-Dong, ZHANG Wei-Ying, YE Li-Hong. Analysis of Phosphatidylcholines (PCs) and Lysophosphatidylcholines (LysoPCs) in Metastasis of Breast Cancer Cells[J]. Progress in Biochemistry and Biophysics,2015,42(6):563-573

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:January 27,2015
Revised:April 29,2015
Accepted:April 30,2015
Online: June 24,2015
Published: June 20,2015

Get Citation

Share

Article Metrics

History