Review: Application of Cryo-Electron Microscopy on Epigenetic Studies
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National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences

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This work was supported by grants from National Basic Research Program of China (2015CB856200), Strategic Priority Research Program (XDB08010100) of the Chinese Academy of Sciences, Key Research Program (KJZD-EW-L05) from The Chinese Academy of Sciences, and The National Natural Science Foundation of China (31230018, 31425007, 31521002)

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    Abstract:

    The architecture and structural details of chromatin assembler, chromatin modifier, and chromatin remodeler, and their complexes with nucleosome and chromatin, are critical for revealing the molecular mechanisms for the establishment, maintenance and regulation of epigenetic information. The fast-developing cryo-electron microscopy (cryo-EM) provides an indispensible tool for the structure determiantion of the huge, multi-subunits, and flexible epigenetic regulation related macromolecular assemblies. In this review, we summarized the recent progresses on EM structural studies of macromolecules and complexes relating to epigenetic regulation.

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LI Cheng-Min, ZHOU Min, LI Xiao-Min, ZHU Ping.Review: Application of Cryo-Electron Microscopy on Epigenetic Studies[J]. Progress in Biochemistry and Biophysics,2015,42(11):1063-1072

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History
  • Received:September 25,2015
  • Revised:September 25,2015
  • Accepted:September 28,2015
  • Online: November 23,2015
  • Published: November 20,2015