MicroRNA Target Mimics (TM) interfere with regulation of miRNA to its target mRNA by competitively binding the miRNA．Previously, we found that Cucumber mosaic virus (CMV) as a vector expressing a TM sequence effectively inhibited the activity or stability of somemiRNAs in plants, thereby impaired their regulation to the targets．However, the miRNAs binding to their TM sequences carried by CMV inhibited accumulation of the virus to some extent. To analyze underlying reason of the inhibition to virus accumulation when a miRNA binds to its TM sequence in CMV genome, we analyzed influence of various miRNA TM sequences in CMV genome on virus accumulation using RNA blotting．Then, using GFP as a reporter gene, effect of the TM sequences on virus accumulation was analyzed by fluorescence microscopy, Western blotting and RNA blotting．Subsequently, using GFP as a reporter gene again, we analyzed effect of the TM sequences on GFP translation．Finally, the influence of the TM sequences on virus negative-strand RNA synthesis was tested using CMV trans-replication system．The results showed that targeting of five plant endogenous miRNA species tested to their TM sequences carried by CMV genome inhibited virus accumulation to a varied extent．Binding of the miRNAs to their TM sequences suppressed translation of the GFP protein and synthesis of the negative strand. Plant endogenous miRNAs targeting to their target mimics carried by viral genome inhibits translation of viral protein and synthesis of negative strand, thus reduces virus accumulation．The data present here provide possibility for developing a new antiviral methods.