Cell migration is an essential progress in multicellular organisms and critical for many processes such as angiogenesis, inflammation, development and wound healing. Besides, cell migration is also involved invasion and metastasis of tumor cells. The heat shock protein 27(HSP27) is one of the most widely studied members of small heat shock protein family, which is ubiquitous in organisms. HSP27 is a multifunctional protein, which is involved in cell migration process by regulating focal adhesion and actin polymerization. In addition, HSP27 could also regulate epithelial mesenchymal transition in the early phase of tumor progression and metastasis. This review focuses on the recent researches about the role of HSP27 in cell migration and related tumor metastasis, and also suggests the potential value of HSP27 in clinical treatment.