Five new and cheap rare earth complexes with larch tannin(LT) have been prepared by the chelating reaction of rare earth (Re³⁺) with larch tannin according the liquid phase synthesis method, and these complexes were characterized with FT-IR, XPS, UV and coordination number determination method. The antifungal activity of Re³⁺-LT against Aspergillus niger, Monascus anka, Phanerochaete chrysosporium and Actinomucor elegans was evaluated in vitro by using the oxford cup and agar dilution assay. It was found that the Re³⁺-LT complexes are effective for these four fungi, and the antifungal activity was in the order of Ce³⁺-LT > Gd³⁺-LT > La³⁺-LT > Nd³⁺-LT > Yb³⁺-LT. The minimum inhibitory concentration of Ce³⁺-LT to these four fungi is 1.6 g·L^-1, 1.6 g·L^-1, 0.8 g·L^-1, and 1.6 g·L^-1, respectively, while the Yb³⁺-LT is 3.2 g·L^-1, 1.6 g·L^-1 3.2 g·L^-1, and 3.2 g·L^-1, respectively. In terms of fugicide, Yb³⁺-LT is the best one and the minimum fugicide concentration of Yb³⁺-LT to these four fungi is 6.4 g·L^-1, 3.2 g·L^-1, 3.2 g·L^-1, and 6.4 g·L^-1, respectively. In addition, the fugicide activity of Nd³⁺-LT and Gd³⁺-LT against Aspergillus niger and Actinomucor elegans is less powerful even they have good antifungal activity.