Proteases play a key role in embryogenesis, immune defense, tissue damage repair, angiogenesis, tumor metastasis and other biological processes related to cell migration. In recent years, the mechanisms of protease-medicated tumor cells invasion and migration have been gradually becoming topic of great interest, but the mechanisms of tumor cells escape immune surveillance, proliferation, migration, invasion and ectopic colonization is still not clear. As a result, study on relevant proteases function and mechanism is of importance. In this paper, we summarized the development of studies on the role of relevant proteases in the migration of tumor cells from the normal physiological functions, so as to provide clues and new ideas on the screening and R&D of novel protease inhibitor drugs to target tumor invasion and migration process.