It’s difficult for biomacromolecules to permeate the barrier of the stratum corneum. As a novel transdermal drug delivery system, microneedles not only greatly promote the transdermal rate and absorption of biological macromolecules, but also possess several advantages like minimal invasiveness, slight pain and convenience. In this paper, the advances in the application of microneedles loaded with biomacromolecule drugs were reviewed in detail, including the promotion of biomacromolecules by individual microneedle arrays (e.g. solid microneedles, hollow microneedles, coated microneedles and soluble microneedles) and combination of microneedles and other pharmaceutic techniques (such as microparticle delivery system), medical devices and intelligent drug-release system. The combinations between microneedle arrays and other technologies have potential to solve the problems that unable for the single microneedle delivery system. In addition, the current problems encountered of microneedle array technique are summarized, and the prospect of microneedles in the field of macromolecular drug delivery is forecasted.