The tumor killing and immune stimulation effects of photothermal therapy using nano graphene oxide(NGO)were studied in vitro and in vivo. For in vitro study, NGO showed a good photothermal conversion effect, increasing temperature under laser irradiation to destroy tumor cells. In addition, NGO could effectively stimulate the production of IL-6 and TNFα by macrophages and enhance the immune response of macrophages to laser-treated tumor cells. For in vivo study, two tumors were inoculated on both sides of the back of mice, serving as the local primary tumor and surrogate distal metastatic tumor. The results showed that the treatment of NGO combined with laser could effectively ablate the treated primary tumor, and inhibit the growth of untreated distal tumor. It suggested that the treatment of NGO combined with laser therapy could stimulate the anti-tumor immune response of the body to attack distal metastases.