The study on tissue development and regeneration is one of the hot spots of life science. Zebrafish has become one of the popular model organisms due to its rapid growth rate, transparent embryos which is easy to observe, and the capacity to regenerate fin, heart, nervous system, etc. During the development and regeneration process of zebrafish, cell proliferation, differentiation, morphogenesis and other metabolic activities occurs，but little is known about the regulatory mechanism among such complex dynamic processes to us. Further studies on these processes have magnificent meanings, for instance, laying a theoretical foundation for the treatment of many diseases, and also promoting the progress of tissue engineering. Previous study has shown that multifold proteins involved in the regulation of tissue development and regeneration. Protein glycosylation is an important post-translational modification, which can affect cell recognition, adhesion, and signal transduction, even lead to inflammation, cancer and other malignant diseases. In recent years, the development of glycomics provides new ideas for developmental biology and regenerative medicine. In this present review, we summarize the role of glycosylation alteration in the development process of zebrafish, and prospect for its future study on regeneration.