Objective: To establish a 3D organoid culture system of mouse fetal liver cell using hyaluronic acid. Method: The fetal liver cells isolated from 12 to 14 days mouse embryos were preliminary screening of 2D liver stem/progenitor cells by Kubota’s Medium (KM) and 3D organoid culture in hyaluronan hydrogel. Result: Fetal liver cells grew as clones in 2D cell culture system. Liver stem/progenitor cell colonies maintained proliferation activity. The albumin and urea levels in supernatant increased significantly, which indicated hepatic functional maturation of the cell colonies in 3D hyaluronic hydrogels. The results of Q-PCR showed that the expression levels of liver stem/progenitor markers, such as AFP, CK19, EpCAM and Prox1, significantly reduced and were close to the expression level in adult mouse liver. Conclusion: In this study, stem-cell derived, functional mouse hepatic organoids in hyaluronan hydrogels was successfully established.