lncRNA alternative splicing refers to the process of cleavage of introns in immature lncRNA and ligation of exons to generate mature lncRNA. Abnormal alternative splicing plays an important role in the development and progression of various diseases. lncRNA alternative splicing is directly involved in the pathogenesis of bladder cancer, colorectal cancer, liver cancer, neuroblastoma, and other tumors, as well as embryonic development, cartilage hair development, and multiple system atrophy. Here, we reviewed the causes, regulatory mechanisms, and research advances in the effects of lncRNA alternative splicing. In addition, two databases related to lncRNA alternative splicing (SpliceMap and LNCediting) are described.