C-type lectins (CTLs) are a family of carbohydrate-binding proteins that share a common structural motif; they are quite conserved evolutionarily from arthropods to mammals and play an essential role in immune responses. Aedes aegypti is a pivotal vector for the dengue virus and expresses more than 30 types of C-type lectins, which are critical for both viral and bacterial infections. A recent study indicates that mosGCTL-3 interacts with a dengue virus-2 envelope protein (DENV-2 E protein) in vivo and in vitro thereby enhancing the infection of A. aegypti. Here, in this report we found another C-type lectin protein, mosGCTL-2 with important functions similar to mosGCTL-3. Results from the phylogenetic tree analysis suggested that there is sequence similarity between mosGCTL-2 and mosGCTL-3, with 43.56% of their amino acid sequences being identical. We constructed Drosophila S2 cell expression system to purify mosGCTL-2. In vitro experiments showed that mosGCTL-2 binds to the DENV-2 E protein through a calcium-dependent manner. The upregulation of mosGCTL-2 was noted in A. aegypti and was important for dengue virus 2 (DENV-2) infection in vivo. These findings suggest that mosGCTL-2 may be a pattern recognition receptor that performs an important function in the infection of A. aegypti by the dengue virus.