Hydrolases are a class of more than 200 individual proteins that catalyze the hydrolysis of a range of unique chemical bonds. However, the inherent disadvantages of natural enzymes, such as variability, high cost, laborious preparation and difficult recovery, greatly limit their practical applications. To overcome these shortcomings, researchers have been devoted to the exploration of hydrolases mimics for a long time. Since the discovery of Fe₃O₄ nanoparticles as peroxidase mimics in 2007, a large number of studies on nanoenzymes have continued to emerge. Compared with natural enzymes, nanoenzymes have the advantages such as simple preparation, large-scale production, strong environmental tolerance, low preparation and storage costs, and reusability. Nanohydrolase are nanomaterials with hydrolase activity,and the hydrolase activity of metal organic frame materials, carbon based nanomaterials and gold nanoparticles has been reported. In recent years, the research field of nano hydrolase has entered a booming period, but so far no one has reviewed nanohydrolase. In this review, we first classifies nanohydrolases according to the different substrates and discusses their catalytic mechanisms, then summarizes the factors affecting the activity of nanohydrolases and the application of nanohydrolases, and finally summarizes and discusses the current challenges and future prospects of nanohydrolases.