Microscopy technology has undergone rapid development and has surpassed the limits of optical diffraction. Currently, it mainly includes stimulated emission depletion microscopy (STED), structured illumination microscopy (SIM), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), minimal photon fluxes (MINFLUX) based on the localization of the minimum number of photons, and a variant of MINFLUX technology combining structured illumination microscopy (SIMFLUX). STORM technology has superiority, and on top of it, there are six multi-color imaging technologies currently available. This article introduces the latest multi-color imaging technology and a three-channel imaging technique implemented using spectral imaging. However, three-channel imaging using spectral imaging has issues such as spectral crosstalk and channel alignment errors. Therefore, relevant optimization algorithm principles are introduced. The article also demonstrates the imaging of COS-7 cells on a three-channel STORM microscopy platform, showcasing the superiority of three-channel STORM microscopy.