1) 中国科学院生物物理研究所,生物大分子国家重点实验室,北京 100101;2) 中国科学院大学,北京 100049;3) 华东理工大学药学院,上海 200237
This work was supported by grants from The National Natural Science Foundation of China (31900893, 91849203), the National Key R&D Program (2017YFA0504000) and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB39000000).
Objective Exercise has been approved as an effective anti-aging approach. However, how exercise affects the organelle-specific redox status of the endoplasmic reticulum (ER) and whether it contributes to ER function and healthy aging are still unknown.Methods We constructed an ER-specific reductive stress C. elegans model that overexpresses ctl-1, a homolog of the mammalian catalase gene, to research the effect of ER reductive stress on aging at the organismal level. We then used the HyperionER probe which responds well to hydrogen peroxide to evaluate the redox status in the ER of body wall muscle during swimming and during aging.Results Our results show that H2O2 in the ER was markedly reduced during aging and the number of body bending, the life span and the stress response ability in Pnfya-1::ctl-1ER::mCherry C. elegans was markedly decreased compared with that in Pnfya-1::ctl-1-MER::mCherry, indicating that ER reductive stress occurs during the aging process and ER reductive stress promotes aging at the organismal level. Both short-term and long-term exercise can increase the oxidative power of the ER in C. elegans, and exercise alleviates the age-related ER reductive stress and promotes healthy aging.Conclusion Our results demonstrate the effect of exercise on ER redox status at the organelle level for the first time and uncover a new mechanism for exercise in delaying aging at the organismal level from the redox point of view, suggesting that maintaining the oxidation power of the ER may be a valuable geroprotective strategy.
WANG Yuan-Yuan, QIAO Xin-Hua, SHI Chang, YE Ao-Jun, GUO Miao-Miao, ZHAO Yu-Zheng, CHEN Chang.Rapid Communications:<. b> Exercise Alleviates ER Reductive Stress and Promotes Healthy Aging[J]. Progress in Biochemistry and Biophysics,2022,49(3):444-453
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