Objective Giving up arises when an animal repeats a behavior to avoid danger or to obtain reward without success. It is a common and fundamental behavior that has been extensively studied in model animals such as mice, but the underlying neural mechanisms have not yet been well elucidated. Zebrafish larvae are important model organisms in neuroscience due to their suitability for whole brain imaging. Negative stimuli such as continuous electric shock have been used to induce giving up behavior in zebrafish, however, whether reward stimuli can induce giving up in zebrafish has not been reported.Methods By presenting larval zebrafish visual stimuli of paramecium, the hunting behavior of zebrafish on virtual food was examined, and the changes of the zebrafish predation frequency and predation duration over time were compared.Results Virtual visual stimulation of food can induce the predatory behavior of zebrafish. After receiving virtual stimuli for 25 min, the predation frequency and single predation duration of over 8 days post fertilization (dpf) zebrafish decreased significantly.Conclusion Our study enriches the paradigm of zebrafish give up behavior. The experimental results show that virtual food stimuli lacking real rewards can induce zebrafish to abandon predation. The current behavioral paradigm provides a new method to study animal give up behavior and the underlying neural mechanisms.