Due to the remarkable properties of fluorine atoms, the physicochemical properties of many molecules can be significantly improved after introducing the fluorine atoms. Therefore, fluorine atom is more and more widely used in pharmaceutical field. In addition, 80% of pharmaceutical compounds are chiral molecules. It should be mentioned that chiral fluorinated alcohols are commonly uslized to bulid chiral pharmacecutical ingredients. Hence, the research exploring the synthesis methods of such structures is of great importance. Asymmetric reduction of fluorinated ketones is a common method for obtaining this structure. Compared with chemical reduction, biocatalytic reduction shows many advantages, such as high enantioselectivity and yield, easy separation and purification. Biocatalysis, especially enzyme-catalyzed reduction of fluorinated ketones, has grown a research hotspot in the field of preparing chiral compounds. At present, biocatalysis methods have some disadvantages. The reaction time is relatively long and the price of biological enzymes and coenzymes is expensive. Accordingly, the scientists should work on shortening the reaction time, recycling biological enzymes and developing more efficient and economical coenzyme regeneration systems. In this paper, the recent development of biocatalytic reduction of fluorine-substituted ketones to fluorinated chiral alcohols is reviewed from the aspects of purified enzyme catalysis and whole-cell catalysis. Moreover, the effect of fluorination on the biocatalytic reduction of ketones is highlighted and the prospect of catalytic reduction method is also put forward in this review.