Alzheimer’s disease (AD) is an unremitting neurodegenerative disorder. Astrocytes play a fundamental role in maintaining the health and function of the central nervous system and memory. The pathological changes in brain of AD are closely related to astrocytes. Increasing evidence indicates that astrocytes undergo both cellular and molecular changes at an early stage in AD. Physical activity can effectively relieve cognitive impairment by regulating astrocytes in AD. In this review, we have summarized the multiple improvement effects on AD by physical activity through the regulation of astrocytes, including improving cell morphology and regulating astrocytes activation, increasing the release of trophic factors, regulating astrocytes phenotype and reducing neuroinflammation, improving glucose and lactate transportation in the brain as well as degrading oxidative stress. The knowledge of this review will pave the way for the prevent and treatment of AD through understanding the mechanisms of the beneficial effects mediated by physical activity through regulating astrocytes in AD.