Substance use disorder (SUD) is a global problem that is crucial to public health and security. Since the effective pharmaceutical agent is still largely lacking, behavioral therapy, psychotherapy, or rehabilitation has been the major approach for the treatment of SUD. In the recent years, there has been growing interest in the effectiveness of non-pharmaceutical intervention for the treatment of neurological and psychological disorders, including acupuncture, deep brain stimulation (DBS), repetitive transcranial magnetic stimulation (rTMS), transcranial direct current stimulation (tDCS) and exercise. From both preclinical and clinical studies, the present review is focused on the studies which investigated the therapeutical potential of acupuncture, DBS, rTMS, tDCS and exercise on the withdrawal duration, craving, drug intake and comorbidity of other behavioral disorders of SUD. The review included the studies involving different substances, including opioids, psychoactive substances, nicotine, alcohol, etc. The results showed that non-pharmacological intervention enabled to significantly reduce craving, drug consumption, prolong abstinence duration and improve cognitive impairment, anxiety and depression induced by substance exposure. Additionally, pharmaceutical treatment is a main therapeutic method, but effective pharmaceutical agents are still largely lacking. Non-pharmaceutical intervention has somewhat effect and mainly used as complementary approach, lacking research on the neurobiological mechanisms. Simultaneous use of pharmaceutical treatment and non-pharmaceutical intervention have better treatment outcomes with the reduced dosage of therapeutic drugs. Although non-pharmaceutical intervention is currently used as a complementary approach, future research is required to specifically examine the neurobiological mechanism underlying the effect of non-pharmaceutical intervention on SUD and improve the technical methods of non-pharmaceutical intervention.