Abstract:Hemoglobin D-Punjab is a common Hb variant in China. This paper describes a new way——Eco RI mapping of the amplified β-globin DNA, for identification of Hb D-Punjab gene. The primers for PCR were designed and synthesized to enzymatically amplify an 144bp fragment which contained an Eco R1 recognition site. So the D-Punjab gene could be easily detected by Eco RI digestion of the amplified sequences on agarose gel electrophoresis owing to a single base change on codon 121. Four Hb DPunjab families from Han, Tibet and Kasak nationalities were analysed by this simple method. This results were also confirmed by oligonucleotide hybridization technique.

Abstract:The authors analyzed the PvuⅡ, HindⅢ and BgⅢ restriction enzyme mappings of seven cases of chronic myeloid leukemia, using a v-abl probe labeled by α-³²P-dCTP. The results showed that the PvuⅡ and HindⅢ mappings of three cases among the seven had been remarkably altered and the multiple changes of PvuⅡ sites had been observed, as compared with the normal subjuct. Therefore, the authors predicted that Ph chromasomal rearrangement in this kind of chronic myeloid leukemia could involve the inside of c-abl oncogene and might be a multiple rearrangement as well.

Abstract:This paper deals with establishing a transcriptional model in which the transcriptional activities of RNA Pol Ⅰ, Ⅱ, Ⅲ in intact nucleus could be determined separately in vitro. With modified Giuffrida method, the nuclei isolated from rat cerebral cortex were purified in good yields ranging from 41 to 52％ and high purity judging with phase-contrast microscopy. According to the method described by Blatti, RNA synthsis were studied under high or low (240 or 50 mmol/L (NH)₂SO₄) ionic strength condition. The inhibited and residual transcription in high-salt system and presence of α-amanitin represented the activities engaged by RNA Pol II and Poi III independently, ihe transcription in low-salt system containing α-amanitin was mainly responsible for RNA Pol I activities. In addition, some factors effecting on the nuclei transcription in vitro were also evaluated. It was concluded that the reported model had advantages being easy to operated, saving materials, and approching cell physiological conditions, it was suitable for studying transcriptional mechanism of cerebral cortex neurons.

Abstract:Sixteen Wistar rats were randomly divided into four groups. One group was put at an ambient temperature of 23℃—25℃ as control. The other groups were exposed in an artificial climatic chamber to DB 40℃, WB 32℃, BG 46℃ and Rh 60％. These rats were decapitated after the rectal temperature rose to 41℃, 42℃ and 43℃ respectively for one hour. Isolation of total RNA from control and heat-shocked rat liver by phenol/chloroform/iscamylalcohol method and fractionation of Poly(A)⁺mRNA by Oligo (dT)-cellulose chromatography were performed. The difference of Poly(A)⁺mRNA between control and heat-shocked rat liver was shown by urea agarose gel electrophoresis. In vitro translation of Poly(A)⁺mRNA in wheat germ system, the synthesis of four major heat shock polypeptides with approximate molecular weights of 71000, 90000, 98000 and 110000 Dalton was observed. The relative amount of major heat shock proteins inereased as the rectal temperature of the heat-exposed rats rose. These results indicated that HSP was atso induced when rats, as a whole, were exposed to a simulated hot enviroment.