Abstract:The intracellular microelectrode recording technique, channel blocker and radioisotope tracer method were used. The burst discharges were evoked by Zn²⁺, no change by sodium channel blocker——Tetrodotoxin, but abolished by calcium channel blocker——Cd²⁺. [⁶⁵Zn²⁺]_i was decreased by Cd²⁺. It is possible, that Zn²⁺, instead of Ca²⁺, goes into cells through calcium channels in the mechanism of BD evoked by Zn²⁺.

Abstract:An algorithm of Stepwise Discriminant Analysis is used in the classification of Plasmodium blood smear. 9 features of the red cell is extracted. 169 red cells (among them 130 cells are normal, 39 contain Plasmodium) are used as training set, 192 red cells (among them 157 cells are normal, 35 contain Plasmodium) as test set. We have done the statistical analyses and got good results. For test set, the false negative rate is 11.4％, the false positive rate is 7.6％.

Abstract:Several polymer supports for sequence analysis were derived from low crosslinked gelatinous and macroporous poly(styrene-divinylbenzene). Three types of functionalized supports have been compared with each other for coupling model peptide and subsequent Edman degradation.The experimental results indicated that under suitable functional group content of about 1.0 m mol NH₂/g of resin, the attaching yields of peptide to macroporous resin were higher than that of the gel type of resin. Macroporous TEPA resin has been demonstrated to be qualified as a polymer support for solid-phase sequence analysis of peptide.

Abstract:Polyacrylamide gel electrophoresis was used to determine esterase isozymes of three strains of Asian corn borer, Ostrinia furnacalis(Guenèe). Two strains were insecticide-resistant and one was susceptible. It is suggested that there were distinct differences in the zymograms of the esterase isozymes among the three strains, especially carboxylesterase. The number of cartoxylesterase bands of both resistant strains was more than that of the susceptible one, but there was no difference in thir cholinesterase. The zymograms were taken as a criterion to differentiate the resistant strain from the susceptible one. The method is suitable for studying resistance of some other agricultural insects and some aspects of methodology were discussed.

Abstract:The effect of three alkylation agents MNNG, ENNG and DMS on HeLa cells grown in culture were examined and the pattern of DNA distribution in cells were analysed by flow cytofluorometry. The results show the cell division was partly inhibited and the rate of DNA synthesis in cells decreased after treatment of MNNG. The proportion of cells in S phase increased with increasing of exposure time to above control levels. DMS shown a similar phenomenon but ENNG only caused a little increase of S phase cells.