Abstract:Human hairs are one of the most easily found forms of biological evidence at crime scenes, Here we report a method isolating DNA from singte hairs and the results of the gene amplification (PCR) from the hairs' DNA. Further studies on the amplified gene fragments are valuable for the forensic identification of individuals.
Abstract:Total gangliosides were isolated from the liver of healthy rabbit, pig, canine, and canine erythrocytes. After high performance thin layer chromategraphy of the total gangliosides, the chromatograms were scanned by TLC scanner, the percentage contents were obtained. On the basis of lipid-bound sialic acid contents, the authors found that canine erythrocyte had the highest GM3 content. GM3 isolated from compacted canine erythrocytes with a yield of 351.0μg per ml and the purity was 92.2％.
Abstract:Using 31P-NMR to study the high energy phosphates of mouse L7811 ascites cells with thymic ceils from 615 line mouse as control. The results showed there was clear differences between them.Even in the late stage of growth, L7811 ascites cells was not completely in an inactive state. For example, the ratio of phosphocreatine (PCr) and α-, β-, γ- adenosine triphosphate (α-, β-, γ-ATP) to phosphate (Pi) was greatly decreased but not completely inactive. The ratio of sugar phosphate (Sp) to phosphocholine (Pcho) plus phosphoethanolamine (PEtn) increased, while glycero(3) phosphocholine (GroPCho) plus glycero(3) phosphoelhano-lamine (Gro PEtn) decreased. Preliminary studies showed that 31P-NMR spectra are sensitive monitor to the progressive changes of the high energy phosphate metabolism of the L7811 ascites growth.
