Abstract:A bicopper (Ⅱ) complex Cu2L2Cl2 with S-methyldithiocarbazate ligand was synthesized and characterized by various physical methods. It has been found that the complex has an activity of inhibition against some moulds bacteria and yeasts. Redox reactions on bilayer lipid membrane (BLM) modified by Cu2L2Cl2 was investigated by cyclic voltammetry method and the action of electron carrier of this complex on BLM was reported.
Abstract:The characterizations of oxygen radicals released from alveolar macrophages (AM) of rats and related measurement conditions were observed with the technique of ESR in this experiment. The results were summarized as follows.1. Of PMA, BCG, ConA and cis-platinum, the first two stimulated AM to produce hydroxyl radical.2. AM stimulated by different agents had different kinetics of the release of the hydroxyl radical. The release peak from AM appeared at min. two after stimulating AM with PMA, with BCG the release peak delayed obviously.3. 5×107AM/ml proved the fit concentration for trapping the signal of the hydroxyl radical from AM in this experiment. 4. There was a great influence of DETAPAC or EDTA on the intension of the signal.
Abstract:Rutin is an effective constituent of some chinese medicinal herb. Interaction study of the medicament with albumin not only can expound the relation of structure and function of the protein and also the action mechanism of the medicament. By using fluorescence polarization and energy transfer technique, we studied on the interaction of human serum albumin (HSA) with medicaments vitamin B6 and rutin. The dissociation constants of medicaments with albumin were obtained. The distances between the number 214 tryptophane and binding sites of vitamin B6 and rutin on the protein are 23.4Å and 24.02Å respectively.
