Abstract:Solid supported bilayer lipid membranes (S-BLM) that posses some properties similar to those of conventional BLM_s can be self-assembled on a freshly cleaved metal wire by a twostep procedure: 1. The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; 2. The new tip of the wire, having become coated with lipid solution, is transferred into 0.1 mol/l KCl. After a few minutes, a stable lipid bilayer forms spontaneously on the tip of wire, as verified by electrical measurements. This paper reports an application of such a supported BLM (S-BLM) in the detection of Pb²⁺ ions. The S-BLM is liquidcrystalline in structure, which makes it amenable to modification for basic studies, as well as for technological applications such as biobensors and molecular electronic devices.

Abstract:Monomeric bR was reconstituted into DMPC vesicles. Absorption spectrum and CD spectrum show bR molecules in DMPC vesicles were in monomeric state. We have measured the effect of pH gradient on photoelectric response of monomeric bR BLM. When the pH in inside container was higher than the pH in outside container and the difference of pH was more than 2, we have observed the change of polarity from positive to negative. Also we have discussed the reason of the change of polarity of photoelectric response.

Abstract:With the help of luminol, fMLP could initiate in peripheral leukocytes a detectable luminol- and fMLP-concentration-dependent chemiluminescent response, which showed mono-or bi-modal kinetics according to different incubation temperatures during the measurement.

Abstract:GST-π was purified from human placenta and its antisurum was raised in rabbits. The antibody IgG was purified and degraded into Fab′ fragment which was conjugated with horseradish peroxidase (HRP) using N-succinimidyl-4-(N-maleimido-methyl) cyclohexane-carboxylate (SMCC) as crosslinking reagent to produce Fab′-HRP conjugate. A sandwich ELISA was established for the microquantitative determination of GST-π. The sentitivity, was 11pg/ tube, which was far more sensitive than the radioimmunoassay so far reported. Using this method, the serum GST-π of 41 cases of normal adult was found to be 1.06+0.94ng/ml. The upper limit of the normal value was 2.5ng/ml. In 30 cases of primary hepatocarcinoma, the level of serum GST-π was 24.4+17.4ng/ml, which was 23 times higher than the normal average value (p＜0.01). The positive rate was 90%. In contrast serum GST-π in 25 cases of chronic hepatitis was determined to be 1.74 + 1.16ng/ml, which was not significantly different from the normal value (p＞0.05). The pseudo-positive rate was 12.0%.