Abstract:Peripheral blood of healthy volunteers was irradiated with ⁶⁰Co in order to study the effects of radiation on superoxide anion radical (O₂^-_·) generation and the inhibitory effects of α₂-macroglobulin (α₂M) blood preparation. It was found that one hour after irradiation, the amount of O₂^-_· released from polymorphonuclear leukocytes (PMNs) was more than that of the non-irradiated group (P＜0.01) and the activities of superoxide dismutase (SOD) in red cells decreased (P＜0.01). When α₂M (138.5 U/ml blood) were added one hour before irradiation, the amount of O₂^-_· released from PMNs were reduced and the activities of SOD in red cells were preserved. These results suggest that one reason of α₂M against radiation may be related to inhibition of O₂^-_· released from PMNs.

Abstract:It was reported that Con A-induced, Luminol-dependent chemiluminescence response of human peripheral lymphocytes might be inhibited by scavengers of reactive oxygen species (ROS), ie, vitamin C, sodium benzoate, mannitol, L-histidine, superoxide dismutase (SOD) and catalase. It was suggested that human lymphocyte chemiluminescence (Ly-CL) is related to the generation of ROS after lymphocytes were stimulated with Con A. ROS participating in human Ly-CL contained ·OH, ¹O₂,H₂O₂, O₂^-_· and so on. The higher the concentraction of Ca²⁺-channel blocker——Verapamil presented, the lower the intensity of human Ly-CL observed. Probably, human Ly-CL depended on increased intracellular [Ca²⁺] of lymphocyte stimulated with antigens or mitogens. These findings propose that human Ly-CL may be an early events of lymphocyte activation.

Abstract:Two androgen related cDNA fragments were recloned into mini-plasmid AN7 (885bp) and then integrated into phage chromosome of lambda Charon 4A-rat liver genomic library by reciprocal recombination. The selected lambda DNA clones with the homologous sequence to the cDNAs were identified by genetic test and dot hybridization.

Abstract:Nitrogen laser pulses (40μJ/pulse, 3Hz) were guided by optic fiber into left cerebral cortex of six rats. The laser evoked potentials(LEP) in right corresponding cortical area were averaged on line, then, the reduced nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), biopterin (BP) and pterin(PT) in six brain areas were simultaneously assayed by the reverse-phase high-performance liquid chromatography(HPLC). The results show that the increased amplitudes of N₂₀₀, P₃₀₀ could be related to the increased metabolism rate of brain energy and the decreased biosynthesis of bioactive substances.

Abstract:The lactose lectins we used in this experiment were extracted from chicken embryo tissues using lactose and urea method. They were further purified with DE-52 cellulose ion exchange chromatography and Dextran Blue-Sepharose 4B affinity chromatography. The purified embryo liver lectin was labeled with ¹²⁵I and its specific radioactivity was 8.6μci/μg. We have utilized the autoradiography combined with scanning electro microscope(SEM-ARG) to observe the lectin receptors on liver cells, kidney ceils and rabbit red cells. The developed silver grains could be seen clearly on these cell surfaces. The silver grains represent the lectin receptor sites on cell surfaces. We found that the grains on liver cells were more than those on cells from other tissues. This result is consistent with the result which has been performed by the experiment of the specificity of chicken lactose lectin. Therefore,it is suggested that the chicken embryo liver lectin has certain specificity.