Abstract:1. Chemical composition of erythrocyte HbA₂ was analyzed in order to clarify the mechanism of hemoglobin A₂ phenomenon. The paper reports the hemoglobin composition of erythrocyte HbA₂.2. The results of two-dimensional electrophoresis showed that erythrocyte HbA₂ contains two Hb components: one corresponds to HbA and the other most probably is hemolysate HbA₂.3. The results of one-dimensional re-electrophoresis showed definitly that erythrocyte HbA₂ is composed of hemolysate HbA₂ and HbA.4. Tentative conclusion: HbA₂ may be combined with HbA in erythrocyte, i.e. there is probably interaction between the two hemoglobins. This is perhaps the possible cause of hemoglobin A₂ phenomenon.

Abstract:In this paper the regulation of the activity of bovine brain 63kD PDE isozyme by phosphorylation and dephosphorylation was studied. The experimental results are as follows: 1. The 63kD PDE isozyme was phosphorylated by the purified bovine brain Ca²⁺/CaM-PK Ⅱ in the presence of Ca²⁺ and CaM, the maximal phosphate incorporation was 1mol/mol subunit of the 63kD PDE isozyme. 2. The phosphorylated 63kD PDE isozyme was dephosphorylated by calcineurin in the presence of Ni²⁺ and CaM. 3. The AC₅₀ for Ca²⁺ of the phosphorylated form of the 63kD PDE isozyme was higher than that of the nonphosphorylated form of the 63kD PDE isozyme.

Abstract:Human P. falciparum hybrid peptide antigen gene has been synthesized by the solidphase phosphoramidite method with ABI381A DNA synthesizer. The gene is 216bp in length and divided into 10 fragments to synthesis. All synthetic fragments were annealed and ligated with T4 DNA ligase. The product of synthetic gene was recombinded with phage M13 mp18 vector and transfected to E. coli JM109. The recombinants were screened by spot hybridization with ³²)P-label synthetic fragment. The sequence of synthetic gene was analysed by dideoxynucleotide chain termination method. The recults showed that synthesized human P. falciparum hybrid antigen gene was identical with the designed one.

Abstract:The active radicals produced from the photolysis of tetra-phenyl-porphyrin(H₂TPP)in benzene have been investigated by spin trapping-ESR technique. The ESR spectrum with 6 lines was detected in the presence of spin trap PBN, it means that spin adduct is [HTPPPBN]^·. The ESR signal with 6 lines was quenched by O₂ and NaI, it can be concluded, therefore, that the photolysis process of H₂TPP was accomplished via triplet excited state.