Abstract:Hydrazido-agarose was prepared and used for the site-specific immobilization of antibody. Antibody was oxidized with sodium periodate, resulting in the production of aldehyde on the carbohydrate moiety. The oxidized antibody was then reacted with the hydrazido-agarose to form stable hydrazone linkages. The method of immobilization resulted in an increased activity and stability of the bound antibody. TNF, γ-IFN and IL-2 were purified by chromatography on immunosorbents prepared with monoclonal antibodies using this method respectively. Their activities were tested.
Abstract:Plant chromosome DNAs released from intact nuclei were analysed in PFGE and it showed that rice chromosome DNAs existed in a form of "240kb-like units" in a releasing condition of this experiments. These units with restriction enzyme digestion were shown to be chromosome-size DNAs up to 1500kb. The possibility of these "240kb-like units" as fundamental organization units of chromosome DNAs was discussed. This technique has been established for a basis of isolating and purifying large DNA fragments, constructing YAC library and restriction enzyme analyzing.
Abstract:A β-1,3-1,4-glucanase is encoded by bglS gene from B. subtilis. The synthesis of the gene product BglS in E. coli is affected by various hosts, different vectors and orientation of the gene fragment.By protein localization analysis and zymogram analysis of active enzyme molecules, we have found that the enzyme has two active molecules (32 kD and 27 kD) in E. coli and the amount of the enzyme in the periplasmic space is very small. The presence of different active products and the specificity of secretion pattern in E.coli may be due to the difference between E.coli and B.subtilis in the protein processing and translocation.
Abstract:Using a luminol-dependent chemiluminescence assay we found tert-butylhydroperoxide to be a strong inhibitor of respiratory burst of the mouse peritoneal macrophage. However, the protection against the inhibition of respiratory burst induced by tert-butylhydrope-roxide was enhanced after the intraperitoneal injection of polysaccharide from Coriolus versicolor. Further investigation exhibited that glutathione peroxidase activity was markly elevated in PSK-treated macrophages, Meanwhile, higher activity of glutathione peroxidase was maintained in PSK-treated macrophages incubated with tert-butylhydroperoxide. These results suggested that the immunological function of macrophage was related to the activity of glutathione peroxidase. Non-specific immunopolysaccharide might prevent macrophage from damage induced by reactive oxygen species by enhancing antioxidation capacity.
