Abstract:Polymorphonuclear leukocytes (PMN)and soluble or particulate matter interact, to produce chemiluminescence (CL)which links to activation of the oxidative metabolism of the cells. While studying the characteristics of the CL induced by antibody-coated crude membrane fragments of K562 tumor cells, the result was observed that PMN were not triggered to a CL response in the absence of antibodies or the fragments. Furthermore, the fact that Cl could be inhibited by colchicine which disturb the integrity of the PMN cytoskeleton, indicate that the cytoskeleton is linked to Fc receptor binding or signal transduction. The luminol-enhanced CL was dependent on the presence of calcium and hydroxyl radicals were involved.

Abstract:The authors propose an improved mathematical model for the secretion system of the hypothalamo-pituitary-thyroid axis, which takes into account both the activation and feedback effects within the axis and the binding of thyroid hormone with proteins. The agreement of the deductions obtained from this model with experimental results is better than any other model appeared.

Abstract:Bovine Cu, Zn-SOD was modified by 2,4-bis (O-methoxypolyethylene glycol)-6-Chloro-S-triazine (activated PEGm₂) in different rates between amino groups of SOD and PEGm₂. The purified PEGm₂-SOD that remained 80％ residual activity with 30％ modified amino groups was obtained. The fluorescence spectrum and circular dichroism of modified SOD were changed; the results of denaturation by GuHCl and of hydrolysis by pepsin showed that PEGm₂-SOD had higher stability than SOD; the results of immunology showed that the immunogenicity and antigenicity of PEGm₂-SOD decreased greatly.