Abstract:The chicken embryo chondrocytes were cultured in the media containing different concentrations of T-2 toxin from Fusarium tricinctum,which was similar in structure with that from Fusarium species found in grains from Kaschin-Beck disease area.When the concentration of the T-2 toxin rose to 0.01 ppm,the collagen microfibrils in extracellular matrix of chicken embryo chondrocytes were significantly decreased,and the activities of cytochrome c oxidase and H+-ATPase of mitochondria were obviously lower than that of the control. The results showed that 0.01 ppm the T-2 toxin induced the changes of structure and function of chicken embryo chondrocytes. The methods reported provided an effective measure to examine if the factor which induced the Kaschin-Beck disease attacked on the target tissue——chondrocytes directly.
Abstract:Acidic and basic fibroblast growth factor were purified from fresh bovine brain by a combination of differential salt precipitation,ion exchange chromatography and heparin-Sepharose affinity chromatography.Both factors can effectively stimulate the incorporation of 3H-TdR into DNA of 3T3 cell. The apparent molecular masses of aFGF is 13.2 kD and bFGF 15.2—15.8kD.The aFGF has an isoelectric point range of 5.0—6.0 and the amino acid composition consistent with that of standard aFGF.
