Abstract:Catalytic antibodies and molecular imprinting are two new trends in artificial imitation of enzymes, and their recent advances have been reviewed on the basis of the host - guest chemistry and supramolecular chemistry.

Abstract:Apolipoprotein J (apo J) has been purified from human plasma HDL and characterized by de Silva et al in 1990. Apo J is a 70kD glycoprotein, comprised of two disulfide-linked subunits designated apo Ja (34-36kD) and apo Jβ(36-39kD) . The sequence of the 427 amino acid residues of apo J was deduced by the cDNA cloning and sequencing. The predicted a helical regions of apo J indicated that three of these could generate amphiphilic α helices, and may be lipid - bind domains in apo J . Apo J mRNA was expressed in relatively high levels in brain，ovary，testis and liver .Apo J is unique among previous characterized human apolipoproteins in its structure and tissue distribution. The function of apo J is thought to be involved in a variety of physiological processes, including bind and transport lipids. regulation of complement function and sperm maturation etc.

Abstract:Through appropriate design and molecular manipulation, mammalian expression vectors could be constructed. Such plasmids, when introduced into suitable mammalian host cells, would effectively express foreign genes of interestes, which constitutes a mammalian gene expression system. Here, the current advances in this field are reviewed.

Abstract:Intercellular adhesion molecule-1 (ICAM-1, CD54) , which belongs to the immunoglobulin superfamily, is one of the important adhesion molecules on the cell surfaces. It can bind rhinovirus and some of the members of the integrin family and involves the developments of inflammation. commen cold, allergy and graft rejection etc. A brief review about the cell distributions , expression regulation, structure ,fuctions and clinical applications of ICAM-1 is described .

Abstract:Diacylglycerol (DAG), as the second massenger to activate protein kinase C (PKC), may be derived not only from hydrolysis of phos-phatidylinositol (PtdIns), but also from hy-drolysis of phosphatidylcholine (PC) , in which phospholipases of the type C and D (PLC andPLD ) participate . Fatty acids (FA), the pro-ducts of phospholipases A2(PLA2) also acti vates PKC. PKC has at least 10 subspeciesand 3 group, namely classical PKC, new PKC and atypical PKC. PKC also participates inregulation of gene expression.

Abstract:Vascular endothelial growth factor (VEGF) with paracrine mechanism has recently been identified . Its growth-promoting activity is specific for vascular endothelial cells in vitro, VEGF also stimulates angiogenesis and increases blood vessel permeability in vivo. Because its bioactivity has a direct bearing on the growth of solid tumors, the researches on VEGF have been payed a good deal of attention and made good progress.

Abstract:The RB gene is located at chromosome 13q14 which spans more than 150kb, with one interal gap, and its product is a phosphoprotein of about 110kD which is constantly expressed in normal retina cells. The RB Protein can specifically bind to SV40 large T, E1A and E7 antigens. The deficiency of the RB gene is the cause of retinoblastoma. Besides, RB gene mutations are detected in osteosarcomas, breast carcinomas, small-cell lung cancer (SCLC) , soft-tissue sarcomas and hematopoietic proliferative disorders. The tumorigenicity can be partially or totally suppressed by introducing the RB gene into the tumor cells.

Abstract:In recent over ten years, owing to the mutual permeation and the coexperiments of biologists and physicists, a new field of biophysical technology was born and has grown up. It not only involves the basic study of cell electromagnetic effect and its mechanism, but also, as a new field in biotechnology, it relates to the wide application of many other fields, such as molecular biology, cellular biology, immunology, medicine, food and agriculture. The newest progresses in this field are summarized.

Abstract:Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been expressed in prokaryotic and eukaryotic cells. Purified to homogeneity, which facilitates to study the structure and function of this factor. Recently, the study of structure and function of rhGM-CSF has been mainly focused on crystal structure, including chemical modification. conformation and stability in solution, mutation and molecular design. The progress in study the structure-function relationship and the mechanism of interaction of GM-CSF with its receptor is discussed.

Abstract:Biological high resolution electron microscopy,a method developed recently, is comparable to X-ray crystallography for determination of high resolution structure of biological macro-molecules. It overcomes some difficulties confronted by X-ray crystallography and can apply directly to the non-crystal biological macro-molecules or to those proteins which can only form two-dimentional crystals. This method contains mainly experimental recording of real structure information and image analysis of the electron micrographs. Several problems which will be encountered in the application of these techniques,e.g.natural structure preservation, radiation damage, poor contrast, and low signal-noise ratio are discussed.

Abstract:The 58kD subunit gene of the human kidney vacuolar H^＋-ATPase has been successfully expressed in E.coli. The fragment of 58kD subunit gene was obtained by polymerase chain reaction (PCR). A clone encoding 58kD subunit was obtained by directly joining PCR product into the plasmid for expression by T7 RNA polymerase (PET). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis of cultured transformantsdemonstrated high expression of 58kD subunit gene. The product of 58kD subunit accounted for 50% of cytoplasmic proteins.

Abstract:A cDNA fragment encoding mature salmon growth hormone (sGH) was successfully amplified by polymerase chain reaction and the restriction recognition sequences were respectively introduced into the 5' end and 3' end of the amplified fragment. The recombinant secretion vector pOsGH153 containing genes coding for the E.coli ompA signal peptide and mature sGH was constructed and confirmed by restriction enzyme analysis and Southern blot hybridization with the oligonucleotide probe. Upon induction of IPTG, the sGH was expressed in E.coli cells and was secreted into the E.coli periplasma.

Abstract:The genes of the 70kD and 33kD subunits of the bovine brain vacuolar proton pump have been successfully expressed in E. Coli. The fragment of the gene of 70kD subunit was obtained by polymerase chain reaction (PCR) from an isolated cDNA encoding the 70kD subunit of the bovine brain vacuolar proton pump. The fragment of the gene of 33kD subunit was also obtained by PCR from the bovine brain cDNA library. Two clones encoding 70kD and 33kD subunits were individually obtained by directly joining the PCR products into PET vector. Sodium dodecyl sulfate (SDS)-poly-acrylamide gel electrophoresis (PAGE)and Western blot analysis of cultured transformants demonstrated high expression.

Abstract:Through the experiment model of the myocardial ischemia/reperfusion and lipoidaemia in rat, both of them obviously induce membrane injury: a drop of membrane phospholipids, an increase in content of free fatty acids, cholesterol and cholesterol/phospholipid ratio, a decrease in membrane lipids fluidity and activity of membrane enzymes (Ca²⁺, Mg²⁺-ATPase). These aterations perhaps related to the increase of lipids hyperoxidation induced by free radical and exchange of lipids.

Abstract:It has been reported that angiotensin Ⅱ(AⅡ) has antiopioid effect. However, the mechanism of this action is still not understood. To evaluate a possible interaction on c-fos protooncogene expression between A Ⅱ and DPDPE (δ receptor agonist) or NDAP (к receptor agonist) , the changing patterns of Fos protein in rat brain (less cortex and cerebellum) tissue induced by them were determined by immunoprecipitation. The results indicated that A Ⅱ at 0.1 μmo/L markedly evoked the Fos protein expression in brain tissue. Meanwhile, both DPDPE (0.1μmol/L) and NDAP (0.1μmol/L) could induce Fos protein increase in brain although their effects were less than that induced by AⅡ.The levels of Fos protein expression by AⅡ plus DPDPE or NDAP are lower than that induced by AⅡ individually. It is suggested that the action on c-fos expression between AⅡ and opioids is antagonistic. And opioids may weaken the evoked action of AⅡ on c-fos expression in the brain.

Abstract:Gangliosides are one of the glycosyl-ceramides, which are particularly abandant in the central nervous system of vertebrates. The content and components of pig brain Gls were detected. There were 0.0894% (W/W) Gls presented in the pig brain. It was about 0. 39% (W/W) of total lipid. The main components were GM1. GD3, GD1a, GD1b and GT1b. GM1 and GD1a of the pig brain were higher than that of human brain.

Abstract:The distribution of Gc subtypes of 201 unrelated. healthy donors in the Han population in Beijing were investigated. Gc^1F is 0.3698,Gc^1S, 0.2812, Gc², 0.3490. There was good agreement between the observed and the expectated value. (Σx²=1.057, P＞0.70).

Abstract:Quantitative analysis of mRNA is an important technique in the study of gene expression regulation. RNase protection assay shows higher sensitivity and easier operation than Dot blot and Nothern blot . The expressions of human β E-globin gene and mouse α-globin gene in transgenic mice were successfully analyzed by RNase protection assay.

Abstract:A direct micro determination of glutathione peroxidase (GSH-PX) activity with spectrophotometry was developed. Conditions of the assay were studied in detail. 10μl blood sample was diluted with distilled water and treated with 10% TCA to remove protein.There was a good linearity between DTNB product and concentration of GSH after 3 minutes enzymatic reaction at the temperature of 37℃ in PH 6.5 solution. The method had higher sensitivity, better reproducibility. It may became useful tool for analyzing GSH-PX in scientific research and clinical work.

Abstract:Specific high titre antisera to TSH were raised in two sheeps injected with 100μg (booster injection. 50μg) highly purified TSH preparation by the multi-site intradermal immunization technique. Blood were bled at two week intervals by cardiac puncture without killing the animals and solution of anti-anemia drug was given to sheeps after each leting blood. The antisera were monitored by TSH RIA Kit. Titres were range from 28×10⁴ to 205×10⁴ and no cross-reaction occured between TSH antisera and human LH, FSH, HCG and all antisera have the avidity more than 10¹⁰L/mol.

Abstract:In vivo naked DNA gene transfer method was used in gene therapy of Parkinson's disease (PD). The complex of rat tyrosine hydroxy-lase (TH) expression plasmid and Lipofectin was injected stereotaxically into striatum of PD rat model. The asymmetric rotational behavior was reduced substantially and quickly. On the third day after injection, drug-induced rotation decreased 50% compared with pretreatment scores. Immunohistochemical staining showed TH-positive nerve cells in striatum of injection side, which indicated that TH gene was uptaked and expressed by nerve cells. These preliminary results have general implications for the application of naked DNA transfer techpique in gene therapy of human neurological disease and specific implications for PD.

Abstract:Using affinity chromatography to eliminate TSH from normal human serum. Preliminary treatment to TSH with 33% saturated ammonium sulfate were coupled to agarose in alkaline condition and packed in column (1.2cm×40cm). Human normal sera were applied to the column at a flow rate of 0.25ml/min, and the column was washed with 0.01mol/L PBS(pH7.4) at a rate of 3ml/min. Materials adsorbed to the immobilized antibodies were eluted with 6mol/L guanidine-HCI. Between experiments, the column was stored in PBS-azide at 4℃.

Abstract:It is known that the dropping rate of optical absorbing value at 300 nm wave length when ribonucleic acid is hydrolysed with ribonuclease is inhibited quantitatively by heparin. Upon this fact. a standard measuring line is plotted when the inhibition is quantitated by known quantity standard heparin. Any unknown can be determined conveniently by comparing with this standard.

Abstract:The following modifications to the polaro-graphic oxygen electrode method for SOD activity determination were made: (1) directly determining at room temperature, (2) introducing standard SOD as activity unit standard,(3) using phosphate buffer as reaction medium, and (4) increasing the pyrogallic acid used. These modifications result in: (1) the abolishment of the bubbles easily produced onthe electrode surface which severely interfere with the determination, (2) increased sensitivity of determination and (3) broadened linear range of SOD activity.