Abstract:Some important peptides of bacterial or fungal origin are synthesized via a template-directed, nucleic-acid-independent nonribosomal mechanism. Recent studies revealed that there is a special kind of macroenzymes, nonribosomal peptide synthetases, which plays a key role in the alternative system. Nonribosomal peptide synthetases are composed of modules, the sequences of which contain the message for peptide synthesis. The understanding of the structure and function of nonribosomal peptide synthetases enables scientists believe that some new peptides can be produced by modifying or recombining these special enzymes.

Abstract:New neural histological methods based on molecular genetic techniques have been used to reveal the delicate structure of Drosophila's neural system. Due to the research on the formation and retrieval of memory, mushroom bodies might play an important role in the formation of long-term memory, and the signal transmission between the dorsal paired medial cells and the mushroom bodies might contribute a lot to the retrieval process of memory. Experiments in different paradigms on the flight-simulator have demonstrated the existence of some advanced functions of the mushroom bodies, so as to provide more evidences to the research on the integration of visual signals.

Abstract:Naturally existing small catalytic RNAs include hammerhead, hairpin, hepatitis delta virus (HDV) and Varkud satellite (VS) ribozymes. The structures of small ribozymes are simple but diversified. Hammerhead ribozyme consists of 3 short helices and a conserved joint chain. Hairpin ribozyme's deeply buried active region is made up of two side-by-side helices. HDV ribozyme folds to a double-knot structure which contains 5 helix arms. VS ribozyme is made up of 5 helix regions and two joint regions. These ribozymes appear to exploit different cleavage mechanisms which depend upon their individual architectures. Metal ions and nucleobases might be candidates for participants in acid/base catalysis. Hammerhead ribozyme has a basic requirement for divalent metal ions, such as Mg²⁺ ions, to complete it's function. However, hairpin ribozyme can be considered as a distinct one that does not require metal ions as cofactors. In the genomic HDV ribozyme, a metal ion functions as a general base and a cytosine residue functions as a general acid.

Abstract:RNA interference (RNAi) is a natural mechanism in organisms in resistance to virus invasion and inhibition of transposon mobility by double-stranded RNA (dsRNA). dsRNA can match with homologous mRNA by base paring to make specific gene inactivation. RNAi was observed in many model organisms, such as Arabidopsis, C.elegans and fungi. Latest study shows that 21～25 nt small interference RNA (siRNA) can mediate specific gene silencing in mammal cells. Being effective and highly specific, RNAi probably becomes a novel technique in knocking gene down and plays important roles in gene function study and gene therapy of diseases.

Abstract:Many fishes produce a repertoire of positively charged antibacterial peptides as part of innate immunity to bacterial invasion. Based on their biochemical and structural properties, fish antibacterial peptides can be classified into four types: amphipathic or hydrophobic α-helical peptides without cysteine; β-sheet peptides with several disulfides bonds; histone-like proteins and glycoproteins. Despite significant variations in length and composition, a common feature of fish antibacterial peptides is that they all have special structures which allow them to bind or disturb the membrane of bacteria in millimolar concentrations. Fish antibacterial peptides are predicted to be translated as prepropeptides that undergo proteolytic cleavage of amino-terminal hydrophobic signal and carboxy-terminal acidic portion to form the mature peptides. Heretofore, several fish antibacterial peptide genes have been cloned and sequencing analyses revealed that most of them were comprised of an open reading frame with four exons and three introns and the upstream region with some consensus binding sequences for transcription factors found in other fish functional genes. Recent data suggest that the details of the antibacterial pathways may vary for different peptides and are assigned to different mechanisms. Some may kill bacteria by forming transmembrane ion channels according to the ‘barrel-stave’ mechanism, and some by disrupting membrane according to the ‘carpet-like’ mechanism. In addition, alternative mechanisms, which include their binding to DNA and their interference with DNA or protein synthesis, have been proposed to explain the antibacterial action of several peptides. The study of fish antibacterial peptides may improve the understanding of peptide-mediated host defence.

Abstract:γ-tubulin，which is usually in the form of γ-tubulin small complex (γTuSC) and γ-tubulin ring complex（γTuRC）, targeting to microtubule organizing centers (MTOCs) via a set of γ-tubulin complex binding proteins (GTBPs), is an ubiquitous protein in eukaryotes and plays an important role in the microtubule nucleation and assembly of mitosis spindle. Recent progress in the structure, molecule models and functions of γ-tubulin complexes is reviewed.

Abstract:A set of 7 ku proteins (named cold shock proteins) is strongly induced in response to a rapid decrease in growth temperature when bacteria adapt to temperatures below their growth temperature. They are rich in aromatic and basic amino acids, suggested to function as molecular chaperones and make cell enhance ability to survive freezing after a cold shock treatment. The similarity and difference of cold shock proteins were described in structure, regulation of expression and function etc. In addtion, the value of them for human kind is in mention.

Abstract:In previous study, a novel gene, LRRC4, a member of leucine-rich repeat(LRR) superfamily was cloned. Expression analysis indicated that LRR may play an important role in the central nervous system. To investigate the function and the structure-function relationship of LRRC4, full length coding region was amplified and subcloned into pGEM T Easy vector. Further, the recombinant plasmid, pEGFP-C1/LRRC4, was constructed and transfected transiently into U251 cell. Under the fluorescence microscope, the green fluorescence produced by LRRC4 fusion protein was observed on the cytoplasmic membrane. Consistent to prediction by bioinformatics, this result indicated that product of LRRC4 is a membrane protein. In addition, the recombinant of LRRC4，pGEX-4T-2/LRRC4 and truncated LRRC4 recombinant, pGEX-4T-2/mLRRC4, were constructed and transformed into E.coli BL21. Induced by 0.5 mmol/L IPTG, The band corresponding to fusion protein were observed in SDS-PAGE as expected. Together with bioinformatic analysis of LRRC4 protein, these results establish the basis for functional study of LRRC4.

Abstract:Wild-type AcMNPV (Autographa california multicapsid nucleopolyhedrovirus) induced Spodoptera litura (Sl-zsu-1) cells apoptosis at 10～12 h post infection, but it could undergo productive replication in Spodoptera frugiperda (Sf-9) cells. The AcMNPV anti-apoptotic gene, p35 gene replication and transcription in different cell lines (Sl-zsu-1 and Sf-9) were investigated by Southern dot blot and RT-PCR respectively. Differences for p35 gene to synthesize and transcribe in these two cell lines suggested that it could not significantly function in the non-permissive cell line due to its untimely and insufficient expression. Inoculation of Sl-zsu-1 cells with wild-type SpltMNPV (Spodoptera litura multicapsid nucleopolyhedrovirus) 12 h prior to AcMNPV infection blocked the apoptosis and rescued the AcMNPV productive replication. It is deduced that P49 protein, a P35 homolog encoded by p49 gene in SpltMNPV genome, might accumulate a certain level at 12 h post infection enough to counteract the caspases effect.

Abstract:Microtubule organization in developing rat oocytes were investigated through confocal microscopy. The inductive effects of some factors on microtubule modification were also studied. The results indicated that the meiotic cell cycle progression of rat oocyte is accompanied by extensive rearrangement of the microtubule network of the cell. Microtubule organization was modified by the treatment of oocytes with taxol, staurosporine, or okadaic acid. Rat oocytes released from the oviducts could undergo spontaneous parthenogenetic activation. They could also be induced to pseudo-cleave by cytochalasin B treatment. The microtubule configuration in rat oocytes undergoing spontaneous parthenogenetic activation or pseudo-cleavage was also observed.

Abstract:Preconditioning of the brain with brief ischamia induces tolerance to subsequent lethal periods of ischemia. It has been suggested that the enhancement in large conductance Ca²⁺-activated potassium (BK_Ca) channel activity is involved in the pathogenesis of ischemic neuronal injury. Inside-out configuration of patch clamp techniques were used to investigate the temporal changes in BK_Ca channel activity in CA1 pyramidal neurons acutely dissociated from rat hippocampus at 6 h, 24 h and 48 h following 3 min of brief ischemia. There were no changes in channel unitary conductance and reversal potential after brief ischemia. In contrast, a significant decrease in the channel open probability was observed during the first 24 h following brief ischemia. Kinetic analyses showed that the postischemic suppression of BK_Ca channel activity was due to a prolongation of the closed time since there was no significant change in open time after brief ischemia. It is suggested that the brief ischemia-induced suppression of BK_Ca channel activity may be associated with ischemic tolerance.

Abstract:In order to study the pathogenesis of stress-related mental disorders, Nissl staining,Golgi staining and electron-microscope were used to explore the effects of chronic stress on morphology and structure of hippocampal CA1 and CA3 pyramidal neurons in rats.The results showed that no changes of morphology and structure were observed in hippocampal CA1 pyramidal neurons in stress group. The number of hippocampal CA3 pyramidal neuron of stress group（35.14±3.85）was significantly less than that of control group（38.74±3.54），P＜0.05. The total length of apical dendrite of stress group（155.67 μm±33.32 μm）was significantly shorter than that of control group（195.63 μm±34.61 μm）,P＜0.05. In addition, the ultrastructural changes,including the condensation of the cytoplasm,the reduction of the cell volume,the shrinkage of the nucleus membrane,the degeneration of the mitochodria, and the illegibility of the rough endoplasmic reticulum, were observed in hippocampal CA3 pyramidal neurons in chronic stress rats. It is suggested that the changes of morphology and ultra structure of pyramidal neurons in hippocampal CA3, not in CA1,would underlie the pathophysiology of the stress-related mental disorders.

Abstract:The synaptic connection from rat lateral posterior thalamic nucleus to primary visual cortex is an important part of extrageniculate visual pathway. The short-term plasticity of this synaptic transmission is investigated in vivo by using field potential recording and iontophoresis methods. The field potentials were depressed rigorously by either paired-pulse stimulation or repetitive stimulation at different frequency (10, 20, 50 Hz). Iontophoresis of bicuculline and 2-hydroxy-saclofen decreased the depression degree, while iontophoresis of Ca²⁺ increased the depression degree. Therefore, the short-term plasticity of synaptic transmission in this pathway is affected by many factors, such as: changing the transmitter release level at pre-synaptic terminal and activity of GABAergic receptors. However, when applying APV, the selective antagonist of NMDA receptors, no any obvious change in short-term synaptic plasticity was obtained. The rigorous short-term plasticity in this visual pathway may play a role in the visual attention function, which is related to the lateral posterior thalamic nucleus.

Abstract:RNA editing is a process in which the genetic information of a gene transcript is changed during or after transcription. RNA editing exists extensively in the higher plant mitochondria, and is a necessary step for forming functional proteins. There may be some relationship between RNA editing and cytoplasmic male sterility (CMS), a kind of phenomenon that is closely associated with mitochondrial genome mutations. The research materials were the gametopthyte male sterility line(A), maintainer line(B) and F₁ hybrid(F₁) of HL-type CMS. cDNAs and DNAs of atp6 were obtained from A, B and F₁ by PCR and RT-PCR. Then sequences of cDNAs and DNAs are compared: A, B and F₁ share the same 15 editing sites found in the transcripts of atp6. The restorer gene in F₁ greatly changed the editing frequency of each editing site. So it is suggested that HL-type CMS is associated with RNA editing of atp6.

Abstract:A plasmid encoding green florescent protein (GFP) was first used to test efficiency of electroporation and optimize parameters for electroporation in vivo. GFP plasmid was efficiently delivered into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. While much less GFP positive cells were observed in tumor and GFP expression could only last 6 days but tumors treated with high voltage/short pulse showed about 2.68 fold more GFP positive cells than tumors treated with low voltage/long pulses. The optimized electroporation parameters was used to mediate therapeutic gene transfer into subcutaneous tumors which derived from T739 mice bladder transitional cell carcinoma cell line (BTT-gfp), human mammary carcinoma cell line (MCF-7) and human hepatoma cell line (SMMC 7721-gfp). Those therapeutic genes included immune reaction regulation factors interleukin12, interleukin2 and GM-CSF or anti-angiogenesis factors such as antisense VEGF121cDNA, soluble form of VEGF receptor (sFlk-1) and Tie2 (ExTek). Inhibition of tumor growth and metastasis were observed in T739 mice carried bladder transitional cell carcinoma and nude mice carried either human breast cancer or liver cancer which were treated with multiple transfers of plasmid encoding interleukine12 mediated by electroporation. MCF-7 and SMMC 7721-gfp derived tumor showed sensitive to single anti-angiogenesis gene therapy, yet definite suppression of growth and metastasis of BTT-gfp tumor was resulted from co-tranfer of sFlk-1 and ExTek gene mediated by electroporation. The results suggest that electroporation is a high efficient, safe and economical method for gene transfer in vivo and electro-gene therapy would be a useful method for solid tumor.

Abstract:TnIAP, which can inhibit apoptosis and was cloned from Tn-5B1-4 cells, was highly expressed in Tn-5B1-4 cells. However，SDS-PAGE and Western blot analysis indicated that only small part of recombinant protein was soluble, which was different from the record that most recombinant protein expressed in insect cells were soluble. Activity assays demonstrated that recombinant soluble TnIAP could inhibit the cleavage of Ac-LEHD-AFC by caspase-9 and the activation of HEK293 cytosolic extracts by caspase-9 from cleaving Ac-DEVD-AFC. This result gave the further evidence that the molecular mechanism of apoptosis is evolutionarily conserved through insects to mammals.

Abstract:Glutamine synthetase （E 6.3.1.2） from thermoacidophilic archaebacterium Sulfolobus acidocaldarius was purified to 25 fold by DEAE-Sepharose and Sephacryl S-300 column. The N-terminal amino acids were determined as PGLPKNEHEALEFLKSNNIKWVDLQ, also one consensus sequence of a conserved region TFMPKP(I/L/F)(F/P/Y)(G/R) was found by using alignment of other glutamine synthetase sequences from archaebacteria, a pair of primers were determined according to the above two sequences. The PCR was processed by using S.acidocaldarius genomic DNA as template and a DNA fragment about 780bp PCR product was achieved. After cloning and sequencing of this fragment, the DNA sequence could be translated into a continuous protein sequence which showed high identity to the S.solfataricus GS sequence. After Southern blot of genomic DNA digested by different combination of restriction enzymes using above DNA fragment as probe, a 2.4 kb fragment digested by BamHⅠ/HindⅢ was cloned into pBluescript KS⁺ plasmid, after colony hybridization, the positive was chosen and a 1.5 kb complete glutamine synthetase gene was sequenced, the gene was then cloned into PET3C plasmide and was induced, the activity of GS was determined. The thermostability of this enzyme showed that it is indeed from thermophilic protein.

Abstract:A four-point bending device was used to investigate the effect of stretching on osteoblasts collagen production. Cells were stretched with different strain level and loading time. Cyclic stretching of the cells with a strain magnitude of 500 με resulted in an increased collagen production while collagen synthesis was significantly reduced with a strain magnitude of 1 000 με and 1 500 με (P＜0.01). A new loading method, step increased stretching was also used. Cells were stretched at 500 με firstly, followed by stretching at 1 000 με for the same hours. Then the strain level was raised to 1 500 με and cells were stretched for the same hours. The step increased stretching experiment showed that osteoblasts absorbed proline to synthesize collagen when received an appropriate signal (500 με). When the strain magnitude was raised, that is to say, the mechanical stimulation was turned into an inhibiting signal, intracellular ³H-proline was released into solution again. These results demonstrate that osteoblasts can adjust themselves to adapt the new mechanical stimulation during the step increased stretching course.

Abstract:A total of 360 arbitrary 10-mer oligonucleotide primers were screened using RAPD (random amplified polymorphic DNA) technique on the genome DNA of Norin 8 and its ⁶⁰Co gamma-ray irradiation mutant Norin 8m. One primer OPG18 produced co-dominant polymorphic markers OPG18/972 and OPG18/943 from Norin 8 and Norin 8m. Based on the clones and sequences of the OPG18/972 and OPG18/943, it shows that a 29 bp DNA fragment in the genome DNA of Norin 8m is deleted. The results directly confirmed the fact that ⁶⁰Co gamma-ray irradiation can induced a bigger DNA fragment deletion of plant genome DNA.

Abstract:A new method to construct a phylogeny tree based on whole genome information is introduced. Each gene of an organism is represented by a 17 dimensional vector, each dimension of which relates to one of the 17 COGs(clusters of orthologous groups of proteins) classes. All the vectors of a genome constitute a set. Then Fisher linear discriminant was used to find a set of optimal weights which reflect more accurately the different contribution of the 17 COGs classes to the genome's evolution. That is, under the Fisher criteria, each vector of a genome is linear mapped. After that, the distance between two genomes was represented by the distance between the related two sets constituted by mapped vectors. At last, the distance matrix was used to construct a phylogenetic tree by PHILP software package. Phylogeny trees of 38 and 43 genomes constructed by this method respectively well support the “three primary kingdom” theory of Woese. This method rectifies the shortcoming of other methods which are difficult to compare genomes differring remarkably in genome size. In addition, the method diminishes the distortion on the distances between genomes brought by lateral gene transfer.

Abstract:Nasopharyngeal carcinoma (NPC) is rare in most parts of the world, but prevalent in south China. Recently, UBAP1 gene, which is located in the region of minimal heterozygosity deletion at 9p21.3～22.1 and down-expressed in NPC, has been cloned. The latest results suggest that the UBAP1 gene is the candidate tumor suppressor for NPC. Association study using 5 single nucleotide polymorphisms (SNPs) within UBAP1 gene by means of sequencing was performed in 105 unrelated case subjects and 183 control subjects which matched to the NPC cases on age, sex and residence. Occasionally, a novel SNP has been found, and been submitted to the dbSNP (accession number: ss4384147). Significant result was obtained for one SNP mark (rs1049557), which is resident at 3′ non-translation region of UBAP1 gene; the relative risk of this SNP mark is 1.64 (genotype GG) and 1.31(genotype CG). The result has proved again that UBAP1 gene may play a certain role in occurrence and development of nasopharyngeal carcinoma. The SNP mark rs1049557, considering it's residence, may influence on the expression of UBAP1 gene.

Abstract:To obtain recombinant glial cell line-derived neurotrophic factor receptor alpha1 (GFRα1) and study its biological activity, the cDNA encoding the mature rat GFRα1 was isolated using RT-PCR with total RNA extracted from newborn SD-rat hippocampus tissue. The expression plasmid pET-GFRα1 was constructed by inserting GFRα1 cDNA into plasmid pET-28a(+) containing T7 promoter and transformed into E.coli BL21(DE3).An expression strain BLGFRα1 was selected.SDS-PAGE analysis revealed that the rat GFRα1 protein was highly expressed and accumulated up to 21.5% of the total bacterial proteins in the form of inclusion body after the induction. By Ni²⁺ chelation affinity chromatography, up to 90% GFRα1 protein was purified. Purified and refolded GFRα1 protein could significantly mediate the ability of GDNF to promote the survival and induce the differentiation of PC12 cells.

Abstract:To study the regularities of reticulocyte's membrane shear elastic modulus and membrane viscosity, a model of anemia in rabbits induced by injection of phenylhydrazine was established. Using a new ektacytometry, the small deformation index (DI)_d and the half-time (t_0.5) for deformation relaxation of the reticulocytes under the abnormal physiological conditions were measured. (DI)_d and t_0.5 were respectively put into the reticulocytes membrane shear elastic modulus (E) formula and the membrane viscosity (μ_m) formula. Through calculation, the value of E and μ_m in the process of reticulocytes changing into erythrocytes in vivo for 72 h were obtained. It was found that the membrane shear elastic modulus and membrane viscosity decreased in this process of changing. This finding had some basic theoretical and clinical significance.

Abstract:The human normal liver cells (L-02) were transfected with plasmid pXJ41-neo and pXJ41-PKCα respectively by lipofectamine agent and were selected positive clones by using G418. The analysis of RT-PCR and Western blot showed that the cell model overexpressing PKCα was constructed successfully. In contrast of control cells (LTC) transfected with pXJ41-neo, the PKCα overexpressing cells (LT3) have enhanced growth rate. The RT-PCR analysis showed that the LT3 cells have elevated transciption level of Ha-ras gene and the luciferase assay showed that Ha-ras gene promoter activity increased, in which the prasGL3 plasmid containing Ha-ras promoter was constructed. On the contrary, the BEL-7402 cells (HT6) transfected with antisense PKCα displayed the decrease of growth rate, Ha-ras gene transciption level and Ha-ras promoter activity compared with the control cells (HTC). The results suggested that the effect of PKCα isoform on Ha-ras oncogene expressing was related with the Ha-ras promoter activity. It seems that PKCα play a positive role in Ha-ras gene expressing regulation.

Abstract:The role of oligosaccharin in the induced resistance of wheat Huixianhong to CY29-1 was studied. Meantime, the time course of endogenous NO was detected by ESR. The results revealed that the oligosaccharin coming from soybean could induce the systemic acquired resistance(SAR) and the SAR may be initiated by endogenous NO.

Abstract:The expression of mIL-12 p70 in the supernatant of cultured COS-7 and B16 melanoma cells transfected with pNEgr-mIL-12 and the tumor growth rate in mice after gene-radiotherapy with pNEgr-mIL-12 plasmid and different doses X-irradiation were observed. The expression of mIL-12 p70 in the supernatant of COS-7 and B16 melanoma cells were most prominent after X-irradiation with 1.5～2.0 Gy, and doses as low as 0.05 Gy also showed a stimulatory effect. Time-course studies showed that the expression of mIL-12 p70 in the supernatant of COS-7 cells reached its peak at 4 h after irradiation and a progressive increase in expression of mIL-12 p70 in the supernatant of B16 melanoma cells was observed over the study period of 72 h. In vivo, the injection of pNE-mIL12 recombinat plasmid into tumor followed by local X-irradiation one or three times could inhibit the growth of B16 melanoma implanted in C57BL/6J mice and the surviving days of tumor-bearing mice was delayed. It will provide an experimental basis for planning effective clinical gene-radiotherapy of cancer.

Abstract:Integrin α_Ⅱbβ₃ is a calcium-dependent heterodimeric protein on platelet, and its extracellular part can bind to RGD containing ligands. Here, a type of sensor prepared by transferring NTA-DOGS containing lipid monolayer to a 50 nm thick gold layer deposited on glass slide is reported. The surface binding ability and regeneration of the sensor were characterized by using a synthetic polypeptide P1 containing six histidine and RGD ligand. The specific binding of integrin to RGD ligand and the effect of divalent cations were investigated. The results show that His-tagged protein can be immobilized on the NTA sensor surface with a functional orientation and the results also show that removing of Ca²⁺ bound on low affinity sites or adding of Mn²⁺ can increase the binding ability of integrin.

Abstract:³⁵S-ACaM2 was producted by using ³⁵S-labeled amino acid mixture in E.coli. SDS-PAGE and autoradiograph indicated that high-purified, high-specific radioactivity ³⁵S-ACaM2 was obtained.Electrophoresis character of ³⁵S-ACaM2 is the same as that of unlabeled ACaM2 with Ca²⁺ or EGTA. Dot-blot and NC overlay experiment showed that ³⁵S-ACaM2 could be used to detect calmodulin-binding proteins as a sensitive probe.

Abstract:Dynamic allele-specific hybridization (DASH), a new method for rapid, economical, accurate, repeatable and high-throughput genotyping of single nucleotide polymorphisms (SNP), has been used successfully for genotyping 2 SNPs in 96 samples. Experimentation technology has been optimized. Primer design criteria, assay validation and its advantage have also been discussed.

Abstract:The rodent testis is generally more susceptible to cadmium(Cd)-induced toxicity than the liver. In order to clarify the molecular mechanism of Cd-induced toxicity in testis, Cd-induced metallothionein(MT) gene expression and MT protein accumulation under different time in testis and liver were compared. MT1 and MT2 mRNA levels were determined by reverse transcription-PCR analysis followed by densitometry scanning, and MT was quantitated by the ELISA method. Both MT mRNA and MT were constitutively present in testis as well as the liver. Testis had higher levels of MT1 and MT2 mRNAs in 1 h, 3 h, 6 h and 24 h over control, but MT protein did not increase. Cd exposure increased hepatic MTs mRNA and MT protein. Thus, it is indicate that, although Cd exposure results in increases of MT mRNA in testis, it does not enhance MT synthesis. The inability to induce the metal-detoxicating MT-protein in response to Cd, might account for higher susceptibility of testis to Cd toxicity and carcinogenesis relative to liver.

Abstract:Comparing the gene expression difference between normal cultured cells and serum starvation cells with DD-PCR method，a cDNA fragment, which expresses increase under serum starvation, was obtained. According to this cDNA fragment, the full gene cDNA was obtained primarily by searching and splicing the EST sequences, then was cloned by PCR and sequenced. The size of this gene is 5 429 bp and it codes a peptide containing 791 amino acid residues. It is not homologous with known genes that related to cell cycle. So, this gene may be a new cell cycle-related gene(GenBank: AY050169). It was named as serum inhibition gene (Si-1) according to its expression inhibited under serum existing.

Abstract:Given an undirected graph, the maximum matching problem is to find a subset of mutually non-adjacent edges having the largest number. This problem is a NP-complete and has no effective method. Adleman introduced firstly the DNA computing in 1994, with which the NP-complete problems are likely to be solved. DNA-based algorithm simulates molecular biology structure of DNA by means of molecular biology technological computation. The plasmid DNA contains a specially inserted series of DNA sequence segments, each of which is bordered by a characteristic pair of restriction enzyme sites, the DNA sequence segments of this series were used to represent the vertices of the graph. The solution is reached by applying enzymatic and gel electrophoresis. The DNA solution to the maximum matching problem of an undirected graph based on the plasmid is introduced. On the basis of the experimental method of bio-molecular, the algorithm is an effective and feasible method.

Abstract:One rapid and inexpensive spectrophotometric method was developed to screen rice embryos for lipoxygenase-3-null, based on the oxidative ability of 9-hydroperoxide, the catalytic product of lipoxygenase-3, combined with the specific inhibitor of lipoxygenase, namely nordihydroguaiaretic acid, when purified lipoxygenase-3 or the surface of cut rice single embryo was exposed to emulsions containing linoleate. The 9-hydroperoxides were detected by one color test based on the oxidation of iodide-starch. The optimal experimental conditions for above method had also been surveyed. Compared with the normal monoclonal antibodies method, this new method is more reliable, simple, costs less reagent, which could also be used as the useful tools for breeding storable rice.