2018年第45卷第6期目录
|
|
封面故事:孙明璇,赵荣涛等利用杂交链式反应与氧化石墨烯(GO)猝灭效应设计了一种现场适宜的甲流病毒检测新方法.当存在目标序列时,可触发杂交链式反应,使标记有FAM基团的发夹结构DNA解链并重新杂交形成长链DNA,进而保护FAM荧光基团不被GO猝灭,从而实现甲流病毒的检测.该方法具有等温(室温)、无酶、体系简单、操作简便等优点,具备现场检测应用前景.
(孙明璇,赵荣涛,李 杨,孔 文,杨 益,郭旭东,刘婉莹,宋宏彬,张志红,郝荣章.基于杂交链式(HCR)反应的甲型流感病毒检测技术研究,本期第644~652页)
Cover Story:Rapid point-of-care (POC) detection of influenza A virus is critical for timely and effective flu prevention and control. In this study, we developed a hybridization chain reaction (HCR)-based assay, through coupling HCR reaction with fluorescence quenching by graphene oxide (GO), for the rapid detection of influenza A virus. The target is detected as it triggers HCR, which results in the extension of short DNA chains, protecting the 6-carboxy-fluorescein amidite (FAM) group from GO quenching. The results demonstrate that it could specifically recognize target nucleic acid fragment of influenza A virus from other pathogen, and even from single-base mismatched oligonucleotides. A good linear correlation between fluorescence intensity and the target concentrations ranging from 10 to 40 nmol/L was achieved, with a detection limit of 5 nmol/L. Its detection performance was verified on nasopharyngeal swab samples, which is the first clinical application of HCR-based influenza assay. This HCR-based method shows many advantages including the enzyme-free amplification of nucleic acids, simple reaction system and convenient protocols, suggesting its availability for POC detection.
|
综述与专论
研究快报
研究报告
|
|