2018年第45卷第6期目录

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封面故事:孙明璇,赵荣涛等利用杂交链式反应与氧化石墨烯(GO)猝灭效应设计了一种现场适宜的甲流病毒检测新方法.当存在目标序列时,可触发杂交链式反应,使标记有FAM基团的发夹结构DNA解链并重新杂交形成长链DNA,进而保护FAM荧光基团不被GO猝灭,从而实现甲流病毒的检测.该方法具有等温(室温)、无酶、体系简单、操作简便等优点,具备现场检测应用前景.
(孙明璇,赵荣涛,李 杨,孔 文,杨 益,郭旭东,刘婉莹,宋宏彬,张志红,郝荣章.基于杂交链式(HCR)反应的甲型流感病毒检测技术研究,本期第644~652页)

Cover Story:Rapid point-of-care (POC) detection of influenza A virus is critical for timely and effective flu prevention and control. In this study, we developed a hybridization chain reaction (HCR)-based assay, through coupling HCR reaction with fluorescence quenching by graphene oxide (GO), for the rapid detection of influenza A virus. The target is detected as it triggers HCR, which results in the extension of short DNA chains, protecting the 6-carboxy-fluorescein amidite (FAM) group from GO quenching. The results demonstrate that it could specifically recognize target nucleic acid fragment of influenza A virus from other pathogen, and even from single-base mismatched oligonucleotides. A good linear correlation between fluorescence intensity and the target concentrations ranging from 10 to 40 nmol/L was achieved, with a detection limit of 5 nmol/L. Its detection performance was verified on nasopharyngeal swab samples, which is the first clinical application of HCR-based influenza assay. This HCR-based method shows many advantages including the enzyme-free amplification of nucleic acids, simple reaction system and convenient protocols, suggesting its availability for POC detection.

综述与专论

CRISPR家族新成员:CRISPR-Cpf1周晨晨,刘写写,谢海华,谷峰  [摘要][PDF][HTML]

刺激响应型纳米载体在肿瘤诊疗中的研究进展田浩,赵鹏飞,郑明彬,马爱青,罗震宇,陈世春,鲁澄宇,蔡林涛  [摘要][PDF][HTML]

环状RNA的生物学功能及其在胃肠肿瘤发生中的作用赵乾富,陈仕俊,肖丙秀,郭俊明  [摘要][PDF][HTML]

WRAP53 β调控DNA损伤修复进展姚羽菲,崔博淼,肖丽英,李燕  [摘要][PDF][HTML]

SDHA与肿瘤细胞代谢葛延山,周艳宏  [摘要][PDF][HTML]

apoA-Ⅰ的α螺旋在胆固醇流出中的作用高嘉慧,莫中成,唐朝克  [摘要][PDF][HTML]

诱导多功能干细胞治疗阿尔茨海默病的研究进展王媛媛,鲍晓明,凌云翔,王钦文,徐淑君  [摘要][PDF][HTML]

研究快报

基于杂交链式(HCR)反应的甲型流感病毒检测技术研究孙明璇,赵荣涛,李杨,孔文,杨益,郭旭东,刘婉莹,宋宏彬,张志红,郝荣章  [摘要][PDF][HTML]

研究报告

Sox9在温度依赖型性别决定中的功能初探张海艳,孙伟,周英杰,包海声,蔡晗,钱国英,葛楚天  [摘要][PDF][HTML]

二氢杨梅素通过下调JNK信号拮抗高糖诱导的PC12细胞凋亡吕慧婕,朱责梅,陈维昭,何剑琴,杨丝丝,张恺芳,奉水东,凌宏艳  [摘要][PDF][HTML]

mRNA环结构对蛋白质折叠速率的影响李瑞芳,李宏,郭春阳,杨萨如拉  [摘要][PDF][HTML]

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